Engineering Biology, Synthetic Biology, Biomaterials and Protein Engineering
Engineer Biology for new Materials
“Science, like all human endeavors, is evolutionary.”
– Frances H. Arnold
Chasing the Dream to Find Greater Medical Impact
Prof. Sun lined Kachin Wong up with his research students WANG Ri, PhD in Bioengineering and YANG Zhongguang, PhD in Chemical and Biomolecular Engineering. Both of them are current postdoctoral fellows in CBE. Early this year, they co-founded a biotechnology start-up named SPES Tech to bring about next-generation therapeutics using their hydrogel product “LitGel”. Prof. Sun is the chairman and the principal scientific advisor of the company.
This study illustrates protein topology engineering as a new strategy for designing smart materials. The work is about to be published in Matter, the flagship journal on materials science owned by Cell Press (Yang et al, 2020, Matter, doi.org/10.1016/j.matt.2019.09.013). It has been selected as the weekly recommended paper by Cell Press (6 in total), and has received big attention from public media.
Fei SUN is one of the RSC MSDE Emerging investigators 2020
Engineering Biology, Synthetic Biology Approaches Toward New Materials, Genetically Encoded Click Chemistry, Directed Evolution, Protein Topology Engineering, Optogenetics and Biosensing
Synthetic Biology Approaches Toward Functional Soft Materials
The creation of macromolecular systems with precise control over their structural and functional properties constitutes a fundamental challenge for molecular engineering. Development of the ability to construct complex biomacromolecular architectures will provide a solution to this challenge. In the past few years, my research group at HKUST has been dedicated to a unique synthetic biology approach for designing advanced protein architectures and soft materials. The central idea is that we use artificially designed genes to direct the synthesis of recombinant proteins or protein-like macromolecules in bacterial cells; the resulting proteins self-assemble into high-order structures, eventually leading to the formation of macroscopic materials like hydrogels, for potential applications ranging from 3D cell culturing to tissue regeneration and chemical separation. In addition, the controlled assembly of protein molecules has also provided us with some exciting tools for studying protein phase separation, particularly in neurons—an important biological phenomenon with wide-ranging impact on biological regulation and disease pathogenesis.
Protein Topology Engineering
Those important biomacromolecules in the Central Dogma, including DNA, RNA and protein, are all synthesized by cellular machinery as linear biopolymers, among which some RNAs and proteins may subsequently fold into compact 3D structures and give rise to functions—which are sometimes accompanied with certain chemical modifications. A fundamental question we are trying to tackle is: Can we ask the cellular machinery to synthesize non-linear protein polymers (Protein Topology Engineering)? If so, we may have access to new materials with properties that have never been explored before. We have been working on this fundamental question through the combined use of synthetic biology and genetically encoded click chemistry; such endeavors have already led to the creation of various exciting protein materials, such as photoresponsive protein hydrogels, that have opened up enormous opportunities for materials biology, regenerative medicine and chemical engineering.